The six-year-old tigress had evaded capture in the jungles of the western state of Maharashtra for two years.
Last month wildlife officials deployed perfume in a bid to lure the animal.
Activists had campaigned to save the tiger, but India's Supreme Court said it would not interfere if forest rangers were forced to shoot it.
A team armed with a tranquiliser gun and a firearm was stationed in a vehicle on a road where the tigress, known as T-1, had been spotted by villagers, a Forestry Department statement said.
A patrol team went to an area near Borati village on Friday evening, after receiving reports of the tigress' presence.
A tranquilliser dart was fired at the animal. Once hit, the tigress charged the patrol's vehicle and she was killed by a single shot from a distance of between eight and 10 metres (26-33 ft), the statement said.
A post-mortem will now be performed on the beast.
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