Flavia spent 43 years living alone in her enclosure at Cordoba Zoo, southern Spain and passed away last week.
Animal rights groups had made several attempts to try and have Flavia moved so she could be homed with other elephants, but were unable to succeed in time.
The elephant's health had been deteriorating for several months, and she was said to suffer from depression, The Local reports.
She collapsed in her enclosure on Friday, and after she was unable to get to her feet, she was euthanized.
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